|
|
| Acceso al texto completo restringido a Biblioteca INIA Las Brujas. Por información adicional contacte bibliolb@inia.org.uy. |
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas. |
Fecha : |
24/11/2022 |
Actualizado : |
25/11/2022 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Autor : |
LARZÁBAL, J.; YAMANAKA, N.; CERETTA, S.; RODRIGUEZ, M.; STEWART, S. |
Afiliación : |
JHON LARZÁBAL, Magíster en Ciencias Agrarias, Facultad de Agronomía, Universidad de la República, Montevideo, Uruguay; NAOKI YAMANAKA, Biological Resources and Post-harvest Division, Japan International Research Center for Agricultural Sciences (JIRCAS), Tsukuba, Ibaraki, Japan; SERGIO EDUARDO CERETTA SORIA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; MARCELO JULIAN RODRIGUEZ ALONZO, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; SILVINA MARIA STEWART SONEIRA, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay. |
Título : |
Introgression of Asian soybean rust resistant genes into elite soybean lines from Uruguay. |
Fecha de publicación : |
2022 |
Fuente / Imprenta : |
International Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.319-327. doi: https://doi.org/10.1080/09670874.2022.2118894 |
ISSN : |
1366-5863 (online) |
DOI : |
10.1080/09670874.2022.2118894 |
Idioma : |
Inglés |
Notas : |
Article history: Received 03 May 2022, Accepted 23 August 2022, Published online: 11 November 2022. Corresponding author: Silvina Stewart, Instituto Nacional de Investigación Agropecuaria (INIA), Programa Nacional de Cultivos de Secano, Colonia, Uruguay. emailto: sstewart@inia.org.uy -- Funding: This study was partly financially supported by the National Institute for Agricultural Research (INIA) and partly by the Japan International Research Center for Agricultural Sciences (JIRCAS) research project "Development of resilient crops and production technologies." -- Supplementary material can be accessed at http://doi:10.1080/09670874.2022.2118894 |
Contenido : |
ABSTRACT.- Soybean is the main agricultural crop in Uruguay. One of the diseases with the greatest damage potential is Asian soybean rust, with estimated grain yield losses up 80% in the region. Throughout history, different authors have stated the importance of genetic resistance as a fundamental tool to decrease the adverse effects of phytopathogens, leading to agriculture sustainability. The objective of this work was to introduce rust resistance genes into elite lines of INIA's soybean breeding program and to evaluate the enhanced resistance. Introgression of resistant genes was carried out in a greenhouse, through three backcrosses from F1. Two donors: Py7-1-47 and No6-12-B handed by JIRCAS with two resistant genes combinations (Rpp1-b + Rpp5 and Rpp4 + Rpp5, respectively) and three elite lines: SJ10-122-040, SJ10-158-039, and SJ10-173-072 from INIA's breeding program were used. After each backcross, plants carrying both genes were selected with marker assisted selection. Each third backcross was self-pollinated (BC3F2) and single plant families were inoculated in the greenhouse. Percentage of rust resistant plants within the introgressed families ranged from 42% to 98%, compared to fully susceptible recurrent parents. © 2022 Informa UK Limited, trading as Taylor & Francis Group |
Palabras claves : |
Biotrophic; Breeding; Phakopsora pachyrhizi; Rpp; Segregation ratio. |
Asunto categoría : |
-- |
Marc : |
LEADER 02882naa a2200265 a 4500 001 1063778 005 2022-11-25 008 2022 bl uuuu u00u1 u #d 022 $a1366-5863 (online) 024 7 $a10.1080/09670874.2022.2118894$2DOI 100 1 $aLARZÁBAL, J. 245 $aIntrogression of Asian soybean rust resistant genes into elite soybean lines from Uruguay.$h[electronic resource] 260 $c2022 500 $aArticle history: Received 03 May 2022, Accepted 23 August 2022, Published online: 11 November 2022. Corresponding author: Silvina Stewart, Instituto Nacional de Investigación Agropecuaria (INIA), Programa Nacional de Cultivos de Secano, Colonia, Uruguay. emailto: sstewart@inia.org.uy -- Funding: This study was partly financially supported by the National Institute for Agricultural Research (INIA) and partly by the Japan International Research Center for Agricultural Sciences (JIRCAS) research project "Development of resilient crops and production technologies." -- Supplementary material can be accessed at http://doi:10.1080/09670874.2022.2118894 520 $aABSTRACT.- Soybean is the main agricultural crop in Uruguay. One of the diseases with the greatest damage potential is Asian soybean rust, with estimated grain yield losses up 80% in the region. Throughout history, different authors have stated the importance of genetic resistance as a fundamental tool to decrease the adverse effects of phytopathogens, leading to agriculture sustainability. The objective of this work was to introduce rust resistance genes into elite lines of INIA's soybean breeding program and to evaluate the enhanced resistance. Introgression of resistant genes was carried out in a greenhouse, through three backcrosses from F1. Two donors: Py7-1-47 and No6-12-B handed by JIRCAS with two resistant genes combinations (Rpp1-b + Rpp5 and Rpp4 + Rpp5, respectively) and three elite lines: SJ10-122-040, SJ10-158-039, and SJ10-173-072 from INIA's breeding program were used. After each backcross, plants carrying both genes were selected with marker assisted selection. Each third backcross was self-pollinated (BC3F2) and single plant families were inoculated in the greenhouse. Percentage of rust resistant plants within the introgressed families ranged from 42% to 98%, compared to fully susceptible recurrent parents. © 2022 Informa UK Limited, trading as Taylor & Francis Group 653 $aBiotrophic 653 $aBreeding 653 $aPhakopsora pachyrhizi 653 $aRpp 653 $aSegregation ratio 700 1 $aYAMANAKA, N. 700 1 $aCERETTA, S. 700 1 $aRODRIGUEZ, M. 700 1 $aSTEWART, S. 773 $tInternational Journal of Pest Management, 2022, vol. 68, issue 4: "Uruguayan Society of Phytopathology (SUFIT): Plant protection for a sustainable agriculture", p.319-327. doi: https://doi.org/10.1080/09670874.2022.2118894
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
|
Registro completo
|
Biblioteca (s) : |
INIA Las Brujas; INIA Treinta y Tres. |
Fecha actual : |
22/07/2018 |
Actualizado : |
28/11/2018 |
Tipo de producción científica : |
Artículos en Revistas Indexadas Internacionales |
Circulación / Nivel : |
Internacional - -- |
Autor : |
WEINROTH, M.D.; SCOTT, H.M.; NORBY, B.; LONERAGAN, G.H.; NOYES, N.R.; ROVIRA, P.J.; DOSTER, E.; YANG, X.; WOERNER, D.R.; MORLEY, P.S.; BELK, K.E. |
Afiliación : |
M.D. WEINROTH, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); H.M. SCOTT, Universidad de Texas A&M; B. NORBY, Universidad de Michigan State (MSU); G.H. LONERAGAN, Texas Tech University; N.R. NOYES, Universidad de Colorado State (CSU); PABLO JUAN ROVIRA SANZ, INIA (Instituto Nacional de Investigación Agropecuaria), Uruguay; E. DOSTER, Universidad de Colorado State (CSU); X. YANG, Universidad de California Davis (UCD); D.R. WOERNER, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program); P. S. MORLEY, Universidad de Colorado State (CSU); K. E. BELK, Universidad de Colorado State (CSU)/ Department of Animal Science (Meat Program). |
Título : |
Effects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle. |
Fecha de publicación : |
2018 |
Fuente / Imprenta : |
Applied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18. |
Descripción física : |
This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. |
ISSN : |
0099-2240 |
DOI : |
10.1128/AEM.00610-18 |
Idioma : |
Inglés |
Notas : |
Article history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. |
Contenido : |
ABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome.
© 2018 American Society for Microbiology. MenosABSTRACT.
Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, gen... Presentar Todo |
Palabras claves : |
ANTIBIOTIC RESISTANCE; ANTIMICROBIAL AGENTS; CATTLE; METAGENOMICS; POSTANTIBIOTIC EFFECT. |
Thesagro : |
FEEDLOT. |
Asunto categoría : |
-- L02 Alimentación animal |
URL : |
http://www.ainfo.inia.uy/digital/bitstream/item/12027/1/e00610-18.full.pdf
|
Marc : |
LEADER 03064naa a2200361 a 4500 001 1058828 005 2018-11-28 008 2018 bl uuuu u00u1 u #d 022 $a0099-2240 024 7 $a10.1128/AEM.00610-18$2DOI 100 1 $aWEINROTH, M.D. 245 $aEffects of ceftiofur and chlortetracycline on the resistomes of feedlot cattle.$h[electronic resource] 260 $c2018 300 $cThis is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license. 500 $aArticle history: Received 15 March 2018. / Accepted 24 April 2018. / Accepted manuscript posted online 4 May 2018. OPEN ACCESS. 520 $aABSTRACT. Treatment of food-producing animals with antimicrobial drugs (AMD) is controversial because of concerns regarding promotion of antimicrobial resistance (AMR). To investigate this concern, resistance genes in metagenomic bovine fecal samples during a clinical trial were analyzed to assess the impacts of treatment on beef feedlot cattle resistomes. Four groups of cattle were exposed, using a 2-by-2 factorial design, to different regimens of antimicrobial treatment. Injections of ceftiofur crystalline- free acid (a third-generation cephalosporin) were used to treat all cattle in treatment pens or only a single animal, and either chlortetracycline was included in the feed of all cattle in a pen or the feed was untreated. On days 0 and 26, respectively, pre- and posttrial fecal samples were collected, and resistance genes were characterized using shotgun metagenomics. Treatment with ceftiofur was not associated with changes to ?-lactam resistance genes. However, cattle fed chlortetracycline had a significant increase in relative abundance of tetracycline resistance genes. There was also an increase of an AMR class not administered during the study, which is a possible indicator of coselection of resistance genes. Samples analyzed in this study had previously been evaluated by culture characterization (Escherichia coli and Salmonella) and quantitative PCR (qPCR) of metagenomic fecal DNA, which allowed comparison of results with this study. In the majority of samples, genes that were selectively enriched through culture and qPCR were not identified through shotgun metagenomic sequencing in this study, suggesting that changes previously documented did not reflect changes affecting the majority of bacterial genetic elements found in the predominant fecal resistome. © 2018 American Society for Microbiology. 650 $aFEEDLOT 653 $aANTIBIOTIC RESISTANCE 653 $aANTIMICROBIAL AGENTS 653 $aCATTLE 653 $aMETAGENOMICS 653 $aPOSTANTIBIOTIC EFFECT 700 1 $aSCOTT, H.M. 700 1 $aNORBY, B. 700 1 $aLONERAGAN, G.H. 700 1 $aNOYES, N.R. 700 1 $aROVIRA, P.J. 700 1 $aDOSTER, E. 700 1 $aYANG, X. 700 1 $aWOERNER, D.R. 700 1 $aMORLEY, P.S. 700 1 $aBELK, K.E. 773 $tApplied and Environmental Microbiology, July 2018, vol. 84, no.13, e00610-18.
Descargar
Esconder MarcPresentar Marc Completo |
Registro original : |
INIA Las Brujas (LB) |
|
Biblioteca
|
Identificación
|
Origen
|
Tipo / Formato
|
Clasificación
|
Cutter
|
Registro
|
Volumen
|
Estado
|
Volver
|
Expresión de búsqueda válido. Check! |
|
|